B.Sc. Theses Successfully Defended in the Laboratory of RNA Biochemistry

Due to the GDPR guidelines, the names of the respective students are not listed here, only the individual supervisors (past and present members of the Laboratory) and titles of the respective theses (along with links to them - if available - in the Charles University Digital Repository). The theses are listed according to their their defense date (in descending order). Some of them are written in Czech but all have at least an English abstract.

Zuzana Feketová, Ph.D.

• mTORC1 complex function in regulation of translation initiation
• Aptamers affecting eIF4F subunits

Klára Frydrýšková, Ph.D.

• Protein diversity in human stress granules

Tomáš Mašek, Ph.D.

• tsRNA - biogenesis, regulation and function in gene expression
• miRNA-mediated translational repression
• Polyadenylation as an integral part of gene expression control in higher eukaryotes

Silvia Mrvová, Ph.D.

• Approaches to mTOR pathway inhibition in acute leukemic cell lines

Martin Pospíšek, Ph.D.

• Interactome of the translation initiation factor eIF4E2
• Inhibitors of the eukaryotic translation initiation factor eIF4E1
• Protein synthesis in cellular stress
• Impact of the rRNA modifications on protein synthesis
• Internal ribosome entry site of the hepatitis C virus as a possible target for therapy
• Phosphorylation of eIF4E in human cancer
• Hepatitis C and options of its treatment
• Operational criteria for IRES definition
• Role of ITAFs in viral IRES function
• Algorithms for searching sequence or structural databases of biomolecules
• The role of eIF4E in translation initiation control in yeast
• Study of the transcriptional activity of ribosomal genes using semi-quantitative RT-PCR
• Database of experimentally studied secondary structures located within 5' untranslated regions of viral RNAs
• Methods for modeling secondary structures of nucleic acids and their correlation with biological reality
• The role of protein kinase A in the regulation of meiotic maturation of mammalian oocytes

Michal Sýkora, Ph.D.

• Intrinsic transcription termination by multisubunit RNA polymerases

Václav Vopálenský, Ph.D.

• Post-transcriptional modification of mRNA molecules in viruses of the Poxviridae family
• Non-template activities of DNA/RNA polymerases and their significance
• Synthesis of proteins containing non-canonical amino acids
• Role of RNA helicases in antiviral defense
• Effect of adenosine deaminase acting on RNA on viral infection of eukaryotic cells
• The design of siRNAs and the influence of modifications on their stability and efficiency
• ERK1/2 MAP kinase - structure and interaction partners
• Catalytic function of DNA-dependent RNA polymerases
• Novel hepatitis C virus proteins

Blanka Zámostná, Ph.D.

• Study of the mammalian oncogenic transcription factors in the yeast model

Blanka Zikánová, M.Sc.

• Metabolism of methionine and its role in cellular processes in Saccharomyces cerevisiae