Methodology

  • Measurement of various chlorophyll fluorescence parameters directly on leaves (fluorescence induction kinetics curve - OJIP parameters, OJIP graphical analysis)
  • Gasometric measurement of net photosynthesis rate, transpiration, and stomatal conductance
  • Spectrophotometric determination of chlorophyll and total carotenoid content
  • Measurement of various spectral reflectance indices directly on leaves
  • Spectrophotometric determination of the proline content and the activities of certain enzymes in proline metabolism (P5CS, PDH, OAT)
  • Spectrophotometric determination of the activities of certain antioxidant enzymes (CAT, APX), as well as hydrogen peroxide and ascorbate contents
  • Spectrophotometric determination of membrane lipid peroxidation (MDA content)
  • Conductometric determination of cell membrane damage index
  • Various standard methods for plant growth and production analysis

 

Laboratory Equipment

  • Portable handheld fluorometers OS30P (ADC BioScientific) and FluorPen 100a (Photon Systems Instruments) (for measuring the rapid phase of the chlorophyll fluorescence induction curve)
  • Portable handheld devices PlantPen PRI and PlantPen NDVI (Photon Systems Instruments)
  • UV-VIS spectrophotometers Anthelie Advanced 2 (Secomam) and Evolution 201 (ThermoScientific)
  • Conductometer GRYF 158 (Gryf HB)
  • Automatic recording devices (Testo) for measurements of air temperature and humidity, soil parameters (moisture, salinity, etc.), water oxygen content, irradiance, etc.
  • Other standard laboratory equipment