Methodology and Technical Support

Methodology

• Commonly used molecular biology methods necessary for work with DNA and RNA, including in situ hybridization, reverse membrane hybridization, RT-qPCR, APOT assay, expression profiling of miRNA, methylation analysis, NGS
• Working with tissue cultures
• Techniques of quantitative immunohistochemistry

Laboratory Equipment

• Necessary equipment for PCR – thermocyclers, shaker thermoblocks
• Necessary equipment for tissue cultures – laminar flow boxes, CO₂ incubators, centrifuges, microscopes
• Necessary equipment for electrophoretic separation of DNA, RNA – electrophoresis units, power supplies, blotting apparatus
• Necessary equipment for hybridization – hybridization oven, thermoblocks for microarray or slide hybridization
• Necessary equipment for quantitative pathology assays