Research | Contact | People | Projects | Publications | Collaboration | Teaching | Alumni
Research
Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumor, virally infected, or stressed cells. We are interested firstly in the structure-function relationship, using a wide range of modern methods of research of protein structure, their properties and interactions, and secondly, how we could transfer the gained knowledge into a practical level, besides other things, by designing artificial protein constructs which would help the immune cells to recognize better and eliminate the target cells. For news and more information, please visit the full website of our research group.
Laboratory of Structural Biochemistry of Immune Recognition
Structural research of complexes of NK cell receptors with their ligands
- Used methods:
- Recombinant protein expression within the HEK293 human cell line
- Biophysical characterization of proteins and their interactions (AUC, ITC, MST, DLS, DSF, SPR)
- Protein crystallography, structural mass spectrometry, SAXS, NMR
Research of NK cell receptor arrangement on the surface of a living cell
- Superresolution microscopy, fluorescence microscopy, flow cytometry, single-molecule localization microscopy (SMLM)
Production of fusion constructs of nanobodies with ligands of activation receptors of NK cells and testing their biological activity
- Isolation of NK cells from the blood of healthy donors
- Cytotoxicity tests of NK cells against tumor cell lines
Production of fusion constructs with cytokines or growth factors
- Expression, purification, and study of properties of fusion proteins containing protein hormones or cytokines
Contact
Department of Biochemistry, Faculty of Science, Charles University
Hlavova 2030/8, 128 00 Prague 2, 2st floor, door 204
tel: +420 221 951 272
e-mail: ondrej.vanek@natur.cuni.cz
People
Group leader: Ondřej Vaněk
Research staff: Jan Bláha (presently post-doc stay at EMBL Hamburg), Barbora Kalousková (presently post-doc stay at TU Wien), Ondřej Skořepa (presently post-doc stay at MPI Biochemie Münich)
PhD. students: Celeste de Sousa Santos Abreu, Shiva Nejadebrahim
MSc. students: Nadezda Bragina, Anastassiya Rudenko, Zuzana Urbanová, Jakub Jeníček, Tereza Cibiková
BSc. students: Daniel Hoffman, Andrea Janoušková, Samuel Janík
Projects
Czech Science Foundation, 15-15181S Harnessing soluble forms of NK cell receptors and their ligands for the generation of novel anticancer immunotherapeutics (2015 – 2017) - O. Vaněk
Grant Agency of the Charles University, 161216 Utilization of a structural description of the interaction of human lymphocyte complex NKR-P1 and LLT1 for development of immunoactive fusion therapeutics (2016 – 2018) - J. Bláha / B. Kalousková
Grant Agency of the Charles University, 927916 Impact of NKp30 glycosylation and oligomerization on its complex formation – the key features for NK cells activation (2016 – 2018) - S. Pažický / O. Skořepa
Ministry of Education, Youth, and Sports of the Czech Republic, INTER-COST program, LTC17065 Utilization of integrative molecular biophysical approaches for the functional studies of human natural killer cell receptors and formation of their complexes with tumor ligands (2017 – 2020) - O. Vaněk
Czech Science Foundation, 18-10687S New ligands for old receptors of human natural killer cells: structure, assembly within the immune synapse and potential for therapy (2018 – 2020) - O. Vaněk
Grant Agency of the Charles University, 1740318 Utilization of activation ligands and nanobodies for directed stimulation of NK cells cytotoxicity (2018 – 2019) - D. Cmunt
Grant Agency of the Charles University, 1378219 Quaternary structure of selected human lymphocyte receptors through the looking glass of super-resolution microscopy: What is the biological reality behind the crystal structures? (2019 – 2021) - B. Kalousková
Czech Science Foundation, 20-13029S Novel clinically relevant IL-2 immunotherapeutics for cancer treatment (2020 – 2022) - O. Vaněk (co-investigator)
Grant Agency of the Charles University, 1436020 Structural biochemistry of NK cell complexes NKp44:PCNA and NKp46:Epa1, and utilization of Epa1 for stimulation of NK cells (2020 – 2022) - J. Houserová
Grant Agency of the Charles University, 1436220 Activation of NK cell anti-tumour response using bispecific fusion proteins with nanobodies (2020 – 2022) - C. Abreu
Ministry of Education, Youth, and Sports of the Czech Republic, INTER-COST program, LTC20078 Glycan-dependent regulation of NK cell recognition: structural biology and novel methods of glycopeptide analysis (2020 – 2023) - O. Vaněk
Grant Agency of the Charles University, 318122 Activation receptor NKp80 and its ligand AICL: structure and mutual interaction in solution and on the surface of human NK cells (2022 – 2024) - K. Pazderová / Z. Urbanová
Grant Agency of the Charles University, 358322 Interaction of galectin-3 with the NKp30 receptor and its role in the antitumor immune response (2022 – 2024) - M. Tulpa
Grant Agency of the Charles University, 285423 Novel recombinant immunocytokines to stimulate the anti-tumor immune response (2023 – 2025) - N. Bragina
Grant Agency of the Charles University, 173923 Utilising sortase A for the preparation of polymer immunotherapeutics and determining their antitumor activity (2023 – 2025) - M. Hejl
Czech Science Foundation, 23-08490L Nanoscale organization of NK cell receptor-ligand complexes through the optics of quantitative single-molecule microscopy (2023 – 2026) - O. Vaněk
Czech Science Foundation, 24-12553O A New Vision: Innovative Imaging Approaches for Investigating the Spatial Organization of Immune Receptors and Ligands (2024 – 2027) - O. Skořepa
Czech Science Foundation, 25-18490S Structural immunology of NK cell recognition: from tumor evasion to its eradication (2025 – 2027) - O. Vaněk
Ministry of Education, Youth, and Sports of the Czech Republic, INTER-ACTION program, LUAUS25250 Exploiting molecular mechanisms of natural killer cell recognition for antitumor therapy (2025 – 2028) - O. Vaněk
Publications
2024
Leonard E. K., Tomala J., Gould J. R., Leff M. I., Lin J. X., Li P., Porter M. J., Johansen E. R., Thompson L., Cao S. D., Hou S., Henclova T., Huliciak M., Sargunas P. R., Fabilane C. S., Vaněk O., Kovar M., Schneider B., Raimondi G., Leonard W. J., Spangler J. B. (2024): Engineered cytokine/antibody fusion proteins improve IL-2 delivery to pro-inflammatory cells and promote antitumor activity. JCI Insight 9, e173469. IF 6.3 DOI: 10.1172/jci.insight.173469
2023
Di Carluccio C., Milanesi F., Civera M., Abreu C., Sattin S., Francesconi O., Molinaro A., Vaněk O., Marchetti R., Silipo A. (2023): Tumor carbohydrate associated antigen analogs as potential binders for Siglec-7. Eur J Org Chem 26, e202300644. IF 3.261 DOI: 10.1002/ejoc.202300644
2022
Anderluh M., Berti F., Bzducha-Wróbel A., Chiodo F., Colombo C., Compostella F., Durlik K., Ferhati X., Holmdahl R., Jovanovic D., Kaca W., Lay L., Marinovic-Cincovic M., Marradi M., Ozil M., Polito L., Reina-Martin J. J., Reis C. A., Sackstein R., Silipo A., Švajger U., Vaněk O., Yamamoto F., Richichi B., van Vliet S. J. (2022): Recent advances on smart glycoconjugate vaccines in infections and cancer. FEBS J 289, 4251–4303. IF 5.622 DOI: 10.1111/febs.15909
Vaněk O., Kalousková B., Abreu C., Nejadebrahim S., Skořepa O. (2022): Natural killer cell-based strategies for immunotherapy of cancer. Adv Protein Chem Struct Biol 129, 91-133. IF 5.447 DOI: 10.1016/bs.apcsb.2022.02.001
Remans K., Lebendiker M., Abreu C., Maffei M., Sellathurai S., May M. M., Vaněk O., de Marco A. (2022): Protein purification strategies must consider downstream applications and individual biological characteristics. Microb Cell Fact 21, 52. IF 6.352 DOI: 10.1186/s12934-022-01778-5
Bláha J., Skálová T., Kalousková B., Skořepa O., Cmunt D., Grobárová V., Pazicky S., Poláchová E., Abreu C., Stránský J., Kovaľ T., Dušková J., Zhao Y., Harlos K., Hašek J., Dohnálek J., Vaněk O. (2022): Structure of the human NK cell NKR-P1:LLT1 receptor:ligand complex reveals clustering in the immune synapse. Nat Commun 13, 5022. IF 17.694 DOI: 10.1038/s41467-022-32577-6
2021
Stehlík Š., Henych J., Štenclová P., Král R., Zemenová P., Pangrác J., Vaněk O., Kromka A., Rezek B. (2021): Size and nitrogen inhomogeneity in detonation and laser synthesized primary nanodiamond particles revealed via salt-assisted deaggregation. Carbon 171, 230-239. IF 11.307 DOI: 10.1016/j.carbon.2020.09.026
Zouharová M., Vymětal J., Bednárová L., Vaněk O., Herman P., Vetýšková V., Poštulková K., Lingstaadas P. S., Vondrášek J., Boušová K. (2021): Intrinsically disordered protein domain of human ameloblastin in synthetic fusion with calmodulin increases calmodulin stability and modulates its function. Int J Biol Macromol 168, 1-12. IF 8.025 DOI: 10.1016/j.ijbiomac.2020.11.216
Stehlík Š., Mermoux M., Schummer B., Vaněk O., Kolářová K., Štenclová P., Vlk A., Ledinský M., Pfeifer R., Romanyuk O., Gordeev I., Roussel-Dherbey F., Němečková Z., Henych J., Bezdička P., Kromka A., Rezek B. (2021): Size effects on surface chemistry and Raman spectra of sub-5 nm oxidized high-pressure high-temperature and detonation nanodiamonds. J Phys Chem C 125, 5647-5669. IF 4.177 DOI: 10.1021/acs.jpcc.0c09190
Anderluh M., Berti F., Bzducha-Wróbel A., Chiodo F., Colombo C., Compostella F., Durlik K., Ferhati X., Holmdahl R., Jovanovic D., Kaca W., Lay L., Marinovic-Cincovic M., Marradi M., Ozil M., Polito L., Reina-Martin J. J., Reis C. A., Sackstein R., Silipo A., Švajger U., Vaněk O., Yamamoto F., Richichi B., van Vliet S. J. (2021): Emerging glyco-based strategies to steer immune responses. FEBS J 288, 4746-4772. IF 5.622 DOI: 10.1111/febs.15830
Peleg Y., Vincentelli R., Collins B. M., Chen K., Livingstone E. K., Weeratunga S., Leneva N., Guo Q., Remans K., Perez K., Bjerga G. E. K., Larsen Ø., Vaněk O., Skořepa O., Jacquemin S., Poterszman A., Kjaer S., Christodoulou E., Albeck S., Dym O., Ainbinder E., Unger T., Schuetz A., Matthes S., Bader M., de Marco A., Storici P., Semrau M. S., Stolt-Bergner P., Aigner C., Suppmann S., Goldenzweig A., Fleishman S. J. (2021): Community-wide experimental evaluation of the PROSS stability-design method. J Mol Biol 433, 166964. IF 6.151 DOI: 10.1016/j.jmb.2021.166964
López-Méndez B., Baron B., Brautigam C. A., Jowitt T. A., Knauer S. H., Uebel S., Williams M. A., Sedivy A., Abian O., Abreu C., Adamczyk M., Bal W., Berger S., Buell A. K., Carolis C., Daviter T., Fish A., Garcia-Alai M., Guenther C., Hamacek J., Holková J., Houser J., Johnson C., Kelly S., Leech A., Mas C., Matulis D., McLaughlin S. H., Montserret R., Nasreddine R., Nehmé R., Nguyen Q., Ortega-Alarcón D., Perez K., Pirc K., Piszczek G., Podobnik M., Rodrigo N., Rokov-Plavec J., Schaefer S., Sharpe T., Southall J., Staunton D., Tavares P., Vaněk O., Weyand M., Wu D. (2021): Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study. Eur Biophys J 50, 411-427. IF 2.095 DOI: 10.1016/10.1007/s00249-021-01532-6
Boušová K., Bednárová L., Zouharová M., Vetýšková V., Poštulková K., Hofbauerová K., Petrvalská O., Vaněk O., Tripsianes K., Vondrášek J. (2021): The order of PDZ3 and TrpCage in fusion chimeras determines their properties – a biophysical characterization. Protein Sci 30, 1653-1666. IF 6.993 DOI: 10.1002/pro.4107
Kalousková B., Skořepa O., Cmunt D., Abreu C., Krejčová K., Bláha J., Sieglová I., Král V., Fábry M., Pola R., Pechar M., Vaněk O. (2021): Tumor marker B7-H6 bound to the coiled coil peptide-polymer conjugate enables targeted therapy by activating human natural killer cells. Biomedicines 9, 1597. IF 4.757 DOI: 10.3390/biomedicines9111597
2020
Janisova L., Gruzinov A., Zaborova O. V., Velychkivska N., Vaněk O., Chytil P., Etrych T., Janoušková O., Zhang X., Blanchet C., Papadakis C. M., Svergun D. I., Filippov S. K. (2020): Molecular mechanisms of the interactions of N-(2-hydroxypropyl)methacrylamide copolymers designed for cancer therapy with the blood plasma proteins. Pharmaceutics 12, 106. IF 6.321 DOI: 10.3390/pharmaceutics12020106
Skořepa O., Pažický S., Kalousková B., Bláha J., Abreu C., Ječmen T., Rosůlek M., Fish A., Sedivy A., Harlos K., Dohnálek J., Skálová T., Vaněk O.* (2020): Natural killer cell activation receptor NKp30 oligomerization depends on its N-glycosylation. Cancers 12, 1998. IF 6.639 DOI: 10.3390/cancers12071998
Sumová P., Polanská N., Leštinová T., Spitzová T., Kalousková B., Vaněk O., Volf P., Rohoušová I. (2020): Phlebotomus perniciosus recombinant salivary proteins polarize murine macrophages towards the anti-inflammatory phenotype. Front Cell Infect Microbiol 10, 427. IF 5.293 DOI: 10.3389/fcimb.2020.00427
Vetýšková V., Zouharová M., Bednárová L., Vaněk O., Sázelová P., Kašička V., Vymětal J., Srp J., Rumlová M., Charnavets T., Poštulková K., Reseland J. E., Boušová K., Vondrášek J. (2020): Characterization of AMBN I and II isoforms and study of their Ca2+-binding properties. Int J Mol Sci 21, 9293. IF 5.923 DOI: 10.3390/ijms21239293
Skálová T., Lengálová A., Dohnálek J., Harlos K., Mihalčin P., Kolenko P., Stráňava M., Bláha J., Shimizu T., Martínková M. (2020): Disruption of the dimerization interface of the sensing domain in the dimeric heme-based oxygen sensor AfGcHK abolishes bacterial signal transduction. J Biol Chem 295, 1587-1597. IF 5,157 DOI: 10.1074/jbc.RA119.011574
2019
Sumová P., Šíma M., Kalousková B., Polanská N., Vaněk O., Oliveira F., Valenzuela J. G., Volf P. (2019): Amine-binding properties of salivary yellow-related proteins in phlebotomine sand flies. Insect Biochem Mol Biol 115, 103245. IF 3.827 DOI: 10.1016/j.ibmb.2019.103245
Willen L., Leštinová T., Kalousková B., Sumová P., Spitzová T., Velez R., Domenech E., Vaněk O., Gállego M., Mertens P., Volf P. (2019): Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis. PLoS Negl Trop Dis 13, e0007832. IF 3.885 DOI: 10.1371/journal.pntd.0007832
Vaněk O.*, Celadová P., Skořepa O., Bláha J., Kalousková B., Dvorská A., Poláchová E., Pucholtová H., Kavan D., Pompach P., Hofbauerová K., Kopecký V. Jr., Mesci A., Voigt S., Carlyle J. R. (2019): Production of recombinant soluble dimeric C-type lectin-like receptors of rat natural killer cells. Sci Rep 9, 17836. IF 3.998 DOI: 10.1038/s41598-019-52114-8
2018
Škerlová J., Bláha J., Pachl P., Hofbauerová K., Kukačka Z., Man P., Pompach P., Novák P., Otwinowski Z., Brynda J., Vaněk O.*, Řezáčová P. (2018): Crystal structure of native β-N-acetylhexosaminidase isolated from Aspergillus oryzae sheds light onto its substrate specificity, high stability, and regulation by propeptide. FEBS J 285, 580-598. IF 4.739 DOI: 10.1111/febs.14360
Malý M., Skálová T., Švecová L., Dohnálek J., Bláha J., Vaněk O., Harlos K., Kolenko P. (2018): Paired refinement: Impact of reintegration and rescaling. In: Dragounová K., ed. Sborník 8. Studentské vědecké konference fyziky pevných látek a materiálů FJFI ČVUT. Praha: České vysoké učení technické v Praze, s. 8-10. ISBN 978-80-01-06511-2
2017
Bláha J., Kalousková B., Skořepa O., Pažický S., Novák P., Vaněk O.* (2017): High-level expression and purification of soluble form of human natural killer cell receptor NKR-P1 in HEK293S GnTI- cells. Protein Expr Purif 140, 36-43. IF 1.338 DOI: 10.1016/j.pep.2017.07.016
Stehlík Š., Varga M., Štenclová P., Ondič L., Ledinský M., Pangrác J., Vaněk O., Lipov J., Kromka A., Rezek B. (2017): Ultrathin Nanocrystalline Diamond Films with Silicon Vacancy Color Centers via Seeding by 2 nm Detonation Nanodiamonds. ACS Appl Mater Interfaces 9, 38842-38853. IF 8.097 DOI: 10.1021/acsami.7b14436
Stráňava M., Man P., Skálová T., Kolenko P., Bláha J., Fojtíková V., Martínek V., Dohnálek J., Lengálová A., Rosůlek M., Shimizu T., Martínková M. (2017): Coordination and redox state-dependent structural changes of the heme-based oxygen sensor AfGcHK associated with intraprotein signal transduction. J Biol Chem 292, 20921-20935. IF 4.011 DOI: 10.1074/jbc.M117.817023
2016
Doležal M., Zábranský A., Dostál J., Vaněk O., Brynda J., Lepšík M., Hadravová R., Pichová I. (2016): Myristoylation drives dimerization of matrix protein from mouse mammary tumor virus. Retrovirology 13, 2. IF 3.867 DOI: 10.1186/s12977-015-0235-8
Stráňava M., Martínek V., Man P., Fojtíková V., Kavan D., Vaněk O., Shimizu T., Martínková M. (2016): Structural Characterization of the Heme-based Oxygen Sensor, AfGcHK, its Interactions with the Cognate Response Regulator, and their Combined Mechanism of Action in a Bacterial Two-component Signaling System. Proteins 84, 1375-1389. IF 2.289 DOI: 10.1002/prot.25083
2015
Bláha J., Pachl P., Novák P., Vaněk O.* (2015): Expression and purification of soluble and stable ectodomain of natural killer cell receptor LLT1 through high-density transfection of suspension adapted HEK293S GnTI- cells. Protein Expr Purif 109, 7-13. IF 1.407 DOI: 10.1016/j.pep.2015.01.006
Skálová T., Bláha J., Harlos K., Dušková J., Kovaľ T., Stránský J., Hašek J., Vaněk O., Dohnálek J. (2015): Four crystal structures of human LLT1, a ligand for human NKR-P1, in varied glycosylation and oligomerization states. Acta Crystallogr Sect D Biol Crystallogr 71, 578-591. IF 2.512 DOI: 10.1107/S1399004714027928
Machová I., Snášel J., Dostál J., Brynda J., Fanfrlík J., Singh M., Tarábek J., Vaněk O., Bednárová L., Pichová I. (2015): Structural and functional studies of phosphoenolpyruvate carboxykinase from Mycobacterium tuberculosis. PLoS ONE 10, e0120682. IF 3.057 DOI: 10.1371/journal.pone.0120682
Lynn G. M., Laga R., Darrah P. A., Ishizuka A. S., Balaci A. J., Dulcey A. E., Pechar M., Pola R., Gerner M. Y., Yamamoto A., Buechler C. R., Quinn K. M., Smelkinson M. G., Vaněk O., Cawood R., Hills T., Vasalatiy O., Kastenmüller K., Francica J. R., Stutts L., Tom J. K., Ryu K. A., Esser-Kahn A. P., Etrych T., Fisher K. D., Seymour L. W., Seder R. A. (2015): In vivo characterization of the physicochemical properties of polymer-linked TLR agonists that enhance vaccine immunogenicity. Nat Biotechnol 33, 1201-1210. IF 43.113 DOI: 10.1038/nbt.3371
Collaboration
Academic partners
Dr. Mario Brameshuber, Technical University of Vienna, Austria
prof. Ario de Marco, University Nova Gorica, Slovenia
prof. James R. Carlyle, University Toronto, Canada (in memoriam)
Dr. Jakub Tomala, prof. Jamie Spangler, John Hopkins University, USA
Dr. Jan Dohnálek, IBT CAS CR, BIOCEV
Dr. Michal Pechar, IMC CAS CR
Industry and commercial partners
Merck s.r.o. (2010-2018)
IOCB TECH s.r.o. (2017–2018)
PROGEN Biotechnik GmbH (2021)
Teaching
Advanced Practical Biochemistry - MC250C17E
Advanced Practical Biochemistry for KATA - MC250C03E
Advanced practical course in biochemistry II - MC250C08
Biology for biochemists - MC250P49
Therapeutic proteins - MC250P67
Introduction to knowledge and technology transfer - MS750P02
Management of biochemistry - MC250P50
Defended bachelor theses: 41
Defended master theses: 27
Defended PhD. theses: 3
Alumni
Supervision of bachelor theses
Hájková Alena (2007): Preparation of vector for the recombinant expression of human protein NKR-P1, receptor of natural killer cell
Němcová Petra (2007): Preparation of vector for the recombinant expression of protein NKR-P1A, receptor of rat natural killer cell
Novotná Lenka (2008): Preparation of vector for the recombinant expression of protein NKR-P1B, receptor of rat natural killer cell
Celadová Petra (2008): Molecular cloning and amplification of b and g isoforms of Clr gene, ligand for Nkrp1 receptors of mouse NK cells
Holubová Martina (2008): Molecular cloning and amplification of B, D, and F isoforms of NKR-P1 mouse natural killer cell receptor
Bláha Jan (2010): Optimization of recombinant protein expression in HEK293 cell line
Čuperková Romana (2012): Optimization of Culture Medium for HEK293 Cell Line
Dvorská Anna (2012): Preparation and studies of complex of rat NK cell receptors NKR-P1B and Clrb
Leontovyč Adrian (2012): Preparation of expression vector for production of receptors CD69 and S1P1
Poláchová Edita (2012): Preparation of Expression Vectors That Regulate Cell Cycle and Apoptosis to Optimize Expression System of HEK293 Cell Line
Pucholtová Helena (2012): Preparation of dimeric form of mouse NK cell receptor NKR-P1C
Illéš David (2013): Optimization of purification of recombinant nitrilase from Arthroderma benhamiae
Mikulová Barbora (2013): Preparation of expression vectors for NKp65 and KACL, new members of human NK cell receptor family
Nový Jiří (2013): Preparation of the human NK cell receptor KACL
Petermannová Romana (2013): Production of auxin transporter PIN1 in HEK293 cell line
Slavata Lukáš (2013): Preparation of plasmids for expression of interleukin 2-fusion monoclonal antibody
Kalousková Barbora (2014): Preparation of glycosylated form of human immunoreceptor NKp30
Pažický Samuel (2014): Preparation of expression vectors for receptor NKp30 and its ligands B7-H6 and BAG-6
Skořepa Ondřej (2014): Preparation of lentiviral expression vector with reporter gene
Gáthy Lajos (2015): Recombinant expression and purification of C324S modified nitrilase from Arthroderma benhamiae
Prokopová Tereza (2015): Production and purification of recombinant receptor Clrb
Herynek Štěpán (2017): Recombinant preparation of proliferating cell nuclear antigen (PCNA)
Krejčová Kateřina (2017): Preparation of tumour ligand B7-H6 with coiled-coil tag and verifying of its binding to receptor NKp30
Hausleitner Filip (2018): The influence of S159A mutation on the oligomeric state of human NK cell receptor NKR-P1A
Houserová Jana (2018): Recombinant expression of protein NKp46 and its ligand Epa1
Nepokojová Tereza (2018): Preparation of fusion ligands and evaluation of their binding to NK cell receptors
Runová Alžběta (2018): Preparation of receptor AICL in fusion with Fc fragment of human IgG
Rožová Dominika (2020): Production of interleukin 2 in fusion with monoclonal antibody S4B6
Gabriela Chytrá (2021): Study of the role of N-glycosylation in function of anti-HER2 nanobody
Hejl Maxmilián (2021): Optimization of in vitro assays focused on activation of NK cells
Kozojedová Petra (2021): Preparation of reporter cell line for validation of recombinant protein biological activity
Pazderová Kristýna (2021): Mutagenesis and expression of protein NKp80, the activation receptor of human lymphocytes
Tulpa Matouš (2021): Galectin-3 and its interaction with the natural cytotoxicity receptor NKp30
Bragina Nadezda (2022): Production of recombinant substrates for transpeptidase reaction
Dubanych Yurii (2022): Production and characterization of galectin-3 mutant
Rudenko Anastassiya (2022): Recombinant expression of the mouse NK cell receptor NKR-P1C
Urbanová Zuzana (2022): Recombinant expression of rat NK cell receptor Clr-b in the presence of fluorinated analogues of monosaccharides
Jeníček Jakub (2023): Preparation of the high-affinity binding domain of protein B7-H6
Cibiková Tereza (2024): Anti-CD20 nanobody production and its conjugation to a polymeric carrier
Maksimova Anna Dmitryvna (2024): Production of a soluble form of the human NK cell receptor NKR-P1 and its ligand LLT1
Murajdová Viktória (2024): Optimization of recombinant protein expression in the human cell line HEK293
Supervision of master theses
Hájková Alena (2009): Recombinant expression and studies on receptor hNKR-P1
Němcová Petra (2009): Recombinant expression and studies on rNKR-P1A receptor
Celadová Petra (2010): Preparation of rat NK cell receptors using HEK293T expression system
Bláha Jan (2012): Preparation and study of human lymphocyte receptor LLT1
Dvorská Anna (2014): Structural biology of complex of rat NK cell receptors NKR-P1B and Clrb
Hradilová Iveta (2014): Preparation and characterization of cyanide hydratase from Aspergillus niger and nitrilase from Arthroderma benhamiae
Poláchová Edita (2014): Optimization of HEK293 cell line expression system by regulation of cell cycle and apoptosis
Pucholtová Helena (2014): Production of mouse NK cell receptor NKR-P1C and seeking of its ligand
Zawadová Dorota (2014): Recombinant expression and purification of nitrilase from Neurospora crassa
Nový Jiří (2015): Preparation and study of human NK cell receptor AICL
Petermannová Romana (2015): Preparation of HEK293 cell line expressing auxin transporter PIN7 and testing of inhibitors of auxin transport
Kalousková Barbora (2016): Preparation of human NK cell activation receptor NKp80 and its ligand AICL
Pažický Samuel (2016): Study of NKp30 oligomerization and its interaction with B7-H6
Skořepa Ondřej (2016): Structural studies of rat NK cell receptor NKR-P1B and its ligand Clrb
Abreu de Sousa Santos Celeste (2019): Interaction of Galectin-1 with human NK cell receptors
Cmunt Denis (2019): Preparation of fusion domains of human immunoreceptors for their utilization in immunotherapy
Herynek Štěpán (2019): Structural biology of receptor NKp44 and its ligand PCNA
Houserová Jana (2020): Study of the interaction of receptor NKp46 with adhesin Epa1
Frantová Eliška (2022): Production of IL-2 fusion antibodies and determination of their biological activity
Malá Viktorie (2022): Preparation of mutated forms of protein B7-H6
Bednaříková Kristýna (2023): Production of a novel type of recombinant IL-2 immunocytokine
Hejl Maxmilián (2023): Preparation of a polymeric therapeutic targeting CD20 positive B-lymphomas and NK cells
Tulpa Matouš (2023): The role of N-glycosylation in homooligomerization of natural cytotoxicity receptor NKp30
Chytrá Gabriela (2024): Activating antitumor immune response using bispecific fusion proteins
Otépka Tomáš (2024: Preparation of growth factor TGF-β3 with coiled-coil tag
Dubanych Yurii (2024): Characterization of the interaction of galectin-3 with the natural cytotoxicity receptor NKp30
Pazderová Kristýna (2024): Structural biochemistry of protein NKp80, an activation receptor of human lymphocytes
Supervision of PhD. theses
Bláha Jan (2017): Study of structure and interaction of human lymphocyte receptors
Skořepa Ondřej (2021): Structural biochemistry of human NK cell receptor complex NKp30 and B7-H6
Kalousková Barbora (2022): Harnessing soluble forms of NK cell receptors and their ligands for cancer immunotherapy