Molecular basis of bacterial adherence in CF

Research

Determination of ion transport through CFTR channel in cell lines of airway epithelium
Development of stable mRNA encoding the chloride channel CFTR for cell transfection
Development and testing of mouse model mimicking conditions of cystic fibrosis (CF) lungs
Effect of antibody or glycomimetics on the bacterium (PA, BC) adherence on epithelial cells ex vivo

Contact

Department of Biochemistry, Faculty of Science, Charles University

Hlavova 2030/8, 128 43 Prague 2, 3st floor, door 368

tel: +420 221 951 241

e-mail: bozena.kubickova@natur.cuni.cz

People

Group leader: Dr. Božena Kubíčková

Research staff: Prof. Petr Hodek

Projects

Publications

Kubíčková, B., Hadrabová, J., Vašková, L., Mandys, V., Stiborová, M., Hodek, P.: Susceptibility of airways to Pseudomonas aeruginosa infection: mouse neuraminidase model; Monatsh. Chem., 148, 1993–2002 (2017).

Vašková, L., Nosková, L., Bláhová, B., Wimmerová, M., Dřevínek, P., Kubíčková, B., Stiborová, M., Hodek, P.; Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonas aeruginosa adherence to epithelial cells. Monatsh. Chem., 147, 889-896 (2016).

Nosková, L., Kubíčková, B., Vašková, L., Bláhová, B., Wimmerová, M., Stiborová, M., Hodek, P.; Fluorescent cellular assay for screening agents inhibiting Pseudomonas aeruginosa adherence. Sensors, 15, 1945-1953 (2015).

Cooperation

Department of Medical Microbiology, 2^nd Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic

Department of Pathology, 3^rd Faculty of Medicine, Charles University, Prague, Czech Republic

Institute for Clinical and Experimental Medicine, Prague, Czech Republic

National Institute of Public Health, Prague, Czech Republic

Teaching

Biochemistry I - MC250C01

Practical course in biochemistry laboratory techniques - MC250C08

List of available thesis is here.

Alumni

Kateřina Dostálová, Mgr. 2019