Modulation of key biotransformation enzymes by xenobiotics

Research | Contact | People | Projects | Publications | Cooperation | Teaching | Alumni

Research

• Interaction of food supplements with xenobiotic biotransformation enzymes
• Study on oxidative damage on rat hepatocyte model
• Role of intestinal microbiota in drug and carcinogen metabolism
• Interaction of  selected cosmetic additives with biotransformation enzymes

Contact

Department of Biochemistry, Faculty of Science, Charles University

Hlavova 2030/8, 128 43 Prague 2, 2st floor, door 221

tel: +420 221 951 287

e-mail: hodek@natur.cuni.cz

People

Group leader: Prof. Petr Hodek

Projects

GAČR P303/12/G163 Centre of drug-dietary supplements interactions and nutrigenetics (2012-2018)

GAČR 303/06/0928 Study on structure-function relationships of cytochromes P450 - key enzymes involved in metabolism of drugs and activation of carcinogens (2006-2008)

Publications

2023

Bělonožníková, K.; Stefani Sladkovská, E.; Kavan, D.; Hýsková, V.; Hodek, P.; Šmíd, D.; Ryšlavá, H.; Effect of Agrimonia eupatoria L. and Origanum vulgare L. Leaf, Flower, Stem, and Root Extracts on the Survival of Pseudomonas aeruginosa. Molecules, 2023, Roč. 28, č. 3, DOI: 10.3390/molecules28031019

2022

Vavrová, K.; Indra, R.; Pompach, P.; Heger, Z.; Hodek, P.; The impact of individual human cytochrome P450 enzymes on oxidative metabolism of anticancer drug lenvatinib. Biomedicine & Pharmacotherapy, 2022, Roč. 145, č. January, s.nestránkováno. DOI: 10.1016/j.biopha.2021.112391

2021

Smidova, V.; Michalek, P.; Goliasova, Z.; Eckschlager, T.; Hodek, P.; Adam, V.; Heger, Z.; Nanomedicine of tyrosine kinase inhibitors. Theranostics, 2021, Roč. 11, č. 4, s.1546-1567. DOI: 10.7150/thno.48662

Jáklová, K.; Feglarová, T.; Rex, S.; Heger, Z.; Eckschlager, T.; Hraběta, J.; Hodek, P.; Kolárik, M.; Indra, R.; Apoferritin/vandetanib association is long-term stable but does not improve pharmacological properties of vandetanib. International Journal of Molecular Sciences, 2021, Roč. 22, č. 8, s.nestránkováno. DOI: 10.3390/ijms22084250

Sklenářová, R.; Svrčková, M.; Hodek, P.; Ulrichová, J.; Franková, J.; Effect of the natural flavonoids myricetin and dihydromyricetin on the wound healing process in vitro. Journal of Applied Biomedicine, 2021, Roč. 19, č. 3, s.149-158. DOI: 10.32725/jab.2021.017

Bárta, F.; Dedíková, A.; Bebová, M.; Dušková, Š.; Mráz, J.; Schmeiser, HH.; Arlt, VM.; Hodek, P.; Stiborová, M.; Co-Exposure to Aristolochic Acids I and II Increases DNA Adduct Formation Responsible for Aristolochic Acid I-Mediated Carcinogenicity in Rats. International Journal of Molecular Sciences [online], 2021, Roč. 22, č. 19, s.nestránkováno. DOI: 10.3390/ijms221910479

2020

Dedíková, A.; Bárta, F.; Martínek, V.; Kotalík, K.; Dušková, Š.; Mráz, J.; Arlt, VM.; Stiborová, M.; Hodek, P.; In vivo metabolism of aristolochic acid I and II in rats is influenced by their co-exposure. Chemical Research in Toxicology, 2020, Roč. 33, č. 11, s.2804-2818. DOI: 10.1021/acs.chemrestox.0c00198

Indra, R.; Vavrová, K.; Pompach, P.; Heger, Z.; Hodek, P.; Identification of Enzymes Oxidizing the Tyrosine Kinase Inhibitor Cabozantinib: Cabozantinib Is Predominantly Oxidized by CYP3A4 and Its Oxidation Is Stimulated by cyt b(5) Activity. Biomedicines [online], 2020, Roč. 8, č. 12, s.nestránkováno. DOI: 10.3390/biomedicines8120547

Skotnicová, A.; Boubínová, G.; Boštíková, Z.; Dušková, Š.; Šulc, M.; Kutinová Canová, N.; Mráz, J.; Hodek, P.; Does Dihydromyricetin Impact on Alcohol Metabolism. Physiological Research, 2020, Roč. 69, č. Suplement 4, s.S573-S581. DOI: 10.33549/physiolres.934606

2019

Le, ST.; Malinovská, L.; Vašková, M.; Mező, E.; Kelemen, V.; Borbás, A.; Hodek, P.; Wimmerová, M.; Csávás, M.; Investigation of the Binding Affinity of a Broad Array of l-Fucosides with Six Fucose-Specific Lectins of Bacterial and Fungal Origin. Molecules, 2019, Roč. 24, č. 12, s.nestránkováno. DOI: 10.3390/molecules24122262

Malinovská, L.; Le, ST.; Herczeg, M.; Vašková, M.; Houser, J.; Fujdiarová, E.; Komárek, J.; Hodek, P.; Borbás, A.; Wimmerová, M.; Csávás, M.; Synthesis of beta-d-galactopyranoside-Presenting Glycoclusters, Investigation of Their Interactions with Pseudomonas aeruginosa Lectin A (PA-IL) and Evaluation of Their Anti-Adhesion Potential. Biomolecules, 2019, Roč. 9, č. 11, s.nestránkováno. DOI: 10.3390/biom9110686

2018

Hodek, P., Kubickova, B., Rychnova, J., Dostalova, K., Vyhnalova, K., Mrazkova, J., Mandys, V., Wimmerova, M., Stiborova, M.: Enzymatic desialylation of lung epithelium increases binding of lectin PAIIL, Pseudomonas aeruginosa virulence factor. FEBS OPEN BIO, 2018, Roč. 8, s. 205. 

2010 - 2017

Šulc, M., Hodek, P., Stiborová, M.; The binding affinity of carcinogenic N-nitrosodimethylamine and N-nitrosomethylaniline to cytochromes P450 2B4, 2E1 and 3A6 does not dictate the rate of their enzymatic N-demethylation. Gen. Physiol. Biophys., 29, 175-185 (2010).

Hodek, P., Křížková, J., Frei, E., Singh, R., Arlt, V. M., Stiborová, M.; Impact of beta-naphthoflavone on genotoxicity of food-derived carcinogens. Neuro Endocrinol. Lett., 32 (Suppl), 25-34 (2011).

Naiman, K., Dračínský, M., Hodek, P., Martínková, M., Schmeiser, H.H., Frei, E., Stiborová, M.; Formation, persistence, and identification of DNA adducts formed by the carcinogenic environmental pollutant o-anisidine in rats. Toxicol Sci., 127, 348-359 (2012).

Hodek, P., Koblihová, J., Kizek, R., Frei, E., Arlt, V. M., Stiborová, M.; The relationship between DNA adduct formation by benzo[a]pyrene and expression of its activation enzyme cytochrome P450 1A1 in rat. Environ. Toxicol. Pharmacol., 36, 989-996 (2013).

Stiborová, J., Manhartová, Z., Hodek, P., Adam, V., Kizek, R., Frei, E.; Formation of DNA adducts by ellipticine and its micellar form in rats - A comparative study. Sensors, 14, 22982-22997 (2014).

Hodek, P., Fousová, P., Brabencová, E., Moserová, M., Pávek, P., Anzenbacherová, E., Brotánek, J., Hudeček, J., Frei, E., Stiborová, M.; Effect of dihydromyricetin on benzo[a]pyrene activation in rats. Neuro Endocrinol. Lett., 35 (Suppl. 2), 158-168 (2014).

Stiborová, M., Schmeiser, H.H., Frei, E., Hodek, P., Martínek, V.; Enzymes Oxidizing the Azo Dye 1-Phenylazo-2-Naphthol (Sudan I) and their Contribution to its Genotoxicity and Carcinogenicity. Curr. Drug. Metab., 15, 829-840 (2014).

Stiborová, M., Levová, K., Bárta, F., Dračínská, H., Šulc, M., Hodek, P., Frei, E., Arlt, V. M., Schmeiser, H. H.; Dicoumarol inhibits rat NAD(P)H:quinone oxidoreductase in vitro and induces its expression in vivo. Neuro Endocrinol. Lett., 35 (Suppl. 2), 123-132 (2014).

Stiborová, M., Bárta, F., Levová, K., Hodek, P., Frei, E., Arlt, V.M., Schmeiser, H.H.: The influence of ochratoxin A on DNA adduct formation by the carcinogen aristolochic acid in rats. Arch. Toxicol. 89, 2141-2158 (2015).

Stiborová, M., Bárta, F., Levová, K., Hodek, P., Schmeiser, H. H., Arlt, V. M., Martínek, V.; A mechanism of O-demethylation of aristolochic acid I by cytochromes P450 and their contributions to this reaction in human and rat livers: Experimental and theoretical approaches. Int. J. Mol. Sci., 16, 27561-27575 (2015).

Bárta, F., Levová, K., Hodek, P., Schmeiser, H.H., Arlt, V.M., Stiborová, M.: The effects of heavy metal ions, phthalates and ochratoxin A on oxidation of carcinogenic aristolochic acid I causing Balkan endemic nephropathy. Neuro Endocrinol. Lett., 36 (Suppl. 1), 13–21 (2015).

Kopečná-Zapletalová, M., Krasulová, K., Anzenbacher, P., Hodek, P., Anzenbacherová, E.: Interaction of isoflavonoids with human liver microsomal cytochromes P450: Inhibition of CYP enzyme activities. Xenobiotica, 47, 324-331 (2017).

Bebová, M., Boštíková, Z., Moserová, M., Pávek, P., Anzenbacherová, E., Stiborová M., Hodek P.: Modulation of xenobiotic conjugation enzymes by dihydromyricetin in rats. Monatsh. Chem., 148, 2003–2009 (2017).

Martínek, V., Bárta, F., Hodek, P., Frei, E., Schmeiser, H.H., Arlt, V.M., Stiborová, M.: Comparison of the oxidation of carcinogenic aristolochic acid I and II by microsomal cytochromes P450 in vitro: experimental and theoretical approaches. Monatsh. Chem., 148, 1971-1981 (2017).

Cooperation

Department of Pharmacology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

Center for Advanced Preclinical Imaging, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

Teaching  (Department of Biochemistry, PřF UK)

Biochemistry I - MC250P03I

Advanced Practical Course II - MC250C08

Clinical Biochemistry Practical Course  - MC250C44

The list of the thesis is available here.

Alumni