Laboratory of cystic fibrosis pathogenesis

Research | Contact | People | Projects | Publications | Cooperation | Teaching | Alumni

Research

• Preparation and characterization of hen yolk antibodies against virulence factors of Pseudomonas aeruginosa (PA) and Burkholderia cepacia complex (BC)

• Study of the influence of bacterial (PA, BC) adherence on epithelial cells ex vivo using antibodies and glycomimetics

• Interaction of fluorescently labeled bacterial lectins with epithelial glycoconjugates in human lung sections from healthy subjects and CF patients

Contact

Department of Biochemistry, Faculty of Science, Charles University

Hlavova 2030/8, 128 43 Prague 2, 2st floor, door 221

tel: +420 221 951 287

e-mail: hodek@natur.cuni.cz

People 

Group leader: Prof. Petr Hodek

Research staff: Dr. Božena Kubíčková

Projects

Publications

Thai Le, S., Malinovska, L., Vašková, M., Mező, E., Kelemen, V., Borbás, A., Hodek, P., Wimmerová, M., Csávás, M.: Investigation of the Binding Affinity of a Broad Array of l-Fucosides with Six Fucose-Specific Lectins of Bacterial and Fungal Origin. Molecules, 24, 2262 (2019).

Kubíčková, B., Hadrabová, J., Vašková, L., Mandys, V., Stiborová, M., Hodek, P.: Susceptibility of airways to Pseudomonas aeruginosa infection: mouse neuraminidase model; Monatsh. Chem., 148, 1993–2002 (2017).

Vašková, L., Nosková, L., Bláhová, B., Wimmerová, M., Dřevínek, P., Kubíčková, B., Stiborová, M., Hodek, P.; Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonas aeruginosa adherence to epithelial cells. Monatsh. Chem.147, 889-896 (2016).

Hadrabová, J., Majerová, B., Černá, V., Moserová, M., Holuša, R., Mandys, V., Stiborová, M., Stříž, I., Hodek, P.; Chicken immunoglobulins for prophylaxis: Effect of inhaled antibodies on inflammatory parameters in rat airways. J. Appl. Biomed., 13, 19-22 (2015).

Nosková, L., Kubíčková, B., Vašková, L., Bláhová, B., Wimmerová, M., Stiborová, M., Hodek, P.; Fluorescent cellular assay for screening agents inhibiting Pseudomonas aeruginosa adherence. Sensors, 15, 1945-1953 (2015).

Hodek, P., Trefil, P., Šimůnek, J., Hudeček, J., Stiborová, M.; Optimized protocol of chicken antibody (IgY) purification providing electrophoretically homogenous preparations. Int. J. Electrochem. Sci., 8, 113-124 (2013).

Cooperation

Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 753/5, Brno, Czech Republic

Department of Pharmaceutical Chemistry, University of Debrecen, Egyetem tér 1, Debrecen, Hungary

Department of Medical Microbiology, 2nd Faculty of Medicine of Charles University and Motol Hospital, V Uvalu 84, Prague 5, Czech Republic

Department of Pathology, 3rd Faculty of Medicine of Charles University and University Hospital Královské Vinohrady, Ruská 87, Prague 10, Czech Republic

IKEM, Vídeňská 1958/9, Prague 4, Czech Republic

National Institute of Public Health, Šrobárova 49/48, Prague 10, Czech Republic

Cystic Fibrosis Patients' Club, z.s., Kudrnova 22/95, Prague 5, Czech Republic

Teaching

Biochemistry I - MC250P03I

Advancer Practical Course in Biochemistry II - MC250C08

Practical Course of Clinical Biochemistry - MC250C44

Defended and new thesis can be found here.

Alumni