List of the main methods used in our laboratory.

Working with plant material

  • Model organisms
    • Tobacco (Nicotiana tabacum) cell lines BY-2
    • Arabidopsis thaliana
    • Solanum tuberosum
    • Carnivorous plants
  • Methods
    • cultivation and maintenance of plants/cell cultures
    • protoplast formation
    • transformation by agrobacterium (floral-dip, leaf infiltration, coculture with cell line)
    • transformation with PEG (Polyethylene glycol)
    • Hybridization of Sarracenia, Nepenthes, Pinguicula, sexing of Nepenthes

Work with bacteria

  • cultivation of E. coli and A. thumefacience
  • transformation(elektroporation, heat-shock)

Molecular biological methods

  • cloning: classic or GoldenGate/GoldenBraid cloning systems
  • DNA/RNA isolation, PCR, RT-qPCR, electrophoresis,...
  • library preparation for Next-generation sequencing (NGS), Sanger-sequencing
    • data analysis
  • blotting (Western, Northern, Southern)
  • Co-immunoprecipitation coupled to mass spectrometry (Co-IP + MS/MS)
  • Chromatin immunoprecipitation (ChIP)
  • Determination of enzyme activity
  • Analysis of small RNA (sRNA), transcriptomes (RNA-seq), whole genome sequencing (WGS) -> Next-generation sequencing (NGS) data processing

CRISPR/Cas methods

  • identification of target sites, sgRNA design

Fluorescence microscopy

  • working with a "conventional" fluorescence microscope
  • working with a confocal microscope

Cytometry

  • protoplasts
  • nuclei

Computational methods

  • working with data -> Excel, Python, Bash
  • work with DNA/RNA/proteins in situ -> Geneious
  • work with image data -> ImageJ
  • work with cytometric data -> FlowJo
  • working with CRISPR mutagenesis data -> Geneious, CRISPResso2, Python
  • online tools -> NCBI, TAIR

Introducing

  • SunTag methods (introducing)
  • Modification by Bridge method (planned, was released in 2024)