Location: Viničná 7, 2nd floor, room 203

 

Head of the laboratory: doc. Mgr. Pavel Munclinger, Ph.D. (muncling@natur.cuni.cz)

Lab technician: Zdena Csibreiová (zdenka.csibreiova@natur.cuni.cz)

 

If you want to start working in the lab or have any questions or issues, please contact Pavel Munclinger first.

Laboratory 203 is a facility shared among members of the Animal evolutionary biology unit and some members of other departments.

 

 

The main purpose of the laboratory is to work before PCR (DNA extraction, PCR mixes preparations). Thermoshakers are also needed for some steps in cytogenetic protocols.

 

 

List of current equipment in the lab:

 

Centrifuges

Non-refrigerated, for standard 1.5 or 2 mL tubes (Eppendorf type microtubes)

  • Eppendorf 5424, max 21300 g, 24-place capacity
  • Eppendorf Minispin, max 12100 g, 48-place capacity
  • Hettich Micro 200, max 21382 g, 24-place capacity

 

Refrigerated, various formats

  • Eppendorf 5430R, 1.5 and 2 mL tubes or 15 and 50 mL (Falcon) tubes, max 18213 g for microtubes (7745 g large tubes), 48-place capacity for microtubes (6 large tubes)
  • Sigma 4-16 KS, max 8585 g, two plates in a swing rotor, 96 or 386-well plates, strips, deepwells, DNA(RNA) extraction kits in 96 format

 

Thermoshakers

Two Biosan TS-100, TS-100S (refrigerated), 1.5 and 2 ml tubes

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Thermostats

  • Gallenkamp Hotbox Owen
  • MELAG 80 small thermostat (fits plates, deepwells, 15 ml tubes), rather rough setting of temperature
  • Labnet AccuBlock Thermoblock for 48 tubes

 

Analytical Balance

Scaltec SBC 22. max. 210 g/0,01/0,1 mg.

 

 

Pipetting robot

Eppendorf epMotion 5070, versatile automated equipment
Single and 8-channel 1-50 µL and 20-300 µL heads, 40-1000 µL heads
Holders for 96 and 384-well plates, 1.5 or 2 mL microtubes, QIAGEN elution tubes in blue boxes. Special epMotion tips are needed (you must buy them before your experiment).

Proper training is required before using the device. Training is provided by Pavel Munclinger (muncling@natur.cuni.cz)

 

 

Fluorometer

Qubit 3.0

Ask Genolab for the condition of usage.

 

Fume cupboard (hood), rather weak, not for work with hazardous chemicals

 

Smaller equipment

  • Rotating Mixer for 1.5/2.0, 15 and 50ml tubes
  • Magnetic mixer IKA C-MAG HS 7
  • pH meter Jenway 3510
  • Two Vortexes
  • 4 microcentrifuges (for tubes and strips)

 

Pipettes are not shared. Each team is supposed to use strictly their own pipettes.

 

However, we do have a set of Starlab pipettes for teaching (practical exercises and other subjects, not for individual diploma or Ph.D. projects). If you are interested in using the set for teaching, please contact Pavel Munclinger (muncling@natur.cuni.cz). The set comprises: 5 pipettes 2,0-20 μl, 5 pipettes 20-200 μl, 2 pipettes 100-1 000 μl, 2 pipettes 0,1-2,5 μl, 2 carousels (holders) for 8 pipettes

 

 

 

Access to the lab:
New users always need to contact Pavel Munclinger and register with her before working in the laboratory. Please, make sure that everyone from your group (students, postdocs, and staff members) who works in the laboratory is registered. The lab has chip access. You can have it installed on your ISIC / ITIC card if you fill in a request form for room 203 at Viničná 7 here: http://web.natur.cuni.cz/www/intranet/formulare/form_cip.php.
You need to generate it in PDF format, print it, and get it signed by Pavel Munclinger and your department head. Once your document has all signatures, deliver it to CIT (Information Technology Division). You can use the faculty post to send it there.

 

 

202, combined laboratory of the department of Zoology

Viničná 7, 2nd floor, room 203

See parts of the laboratory for responsible people.

 

A section of Laboratory 202 (right corner and right small room) is designated for electrophoresis, gel documentation, and work with PCR products.

Head of the laboratory part: doc. Mgr. Pavel Munclinger, Ph.D. (muncling@natur.cuni.cz)

Lab technician: Zdena Csibreiová (zdenka.csibreiova@natur.cuni.cz)

If you want to start working in the lab or have any questions or issues, please contact Pavel Munclinger first.

 

List of current equipment in the lab:

Equipment for electrophoresis of DNA fragments in agarose gels, various tanks, trays, casters, and combs.

 

Fume cupboard (hood), mainly for gel casting, equipped with microwave and magnetic mixer

 

Cyclers: four BIO-RAD T100, two MJ Research PTC200 (rather old, nowadays used mainly as simple incubators), Eppendorf Mastercycler pro S, Eppendorf Mastercycler Nexus. Proper training is required before using Eppendorf Mastercycler pro S. Training is provided by Pavel Munclinger (muncling@natur.cuni.cz).

 

Gel documentation room: Nippon Genetics FAS-Digi PRO documentation system, two printers, additional transilluminator (for fast look at your gel or for gel extractions), two thermoblocks, scales AND HL-400 (max 400 g, d=0.1 g).

A section of Laboratory 202 (left corner) is designated for cytogenetic analyses.

 

Head of the laboratory part: RNDr. František Šťáhlavský, Ph.D. (stahlf@natur.cuni.cz)

 

For chromosome preparations using the plate spreading method, the lab is equipped with a stereomicroscope for dissection and several histological heating plates for final slide drying.

 

For FISH (Fluorescence In Situ Hybridization) techniques, the facility includes:

  • Three water baths for incubations at different temperatures
  • A full set of staining containers
  • Magnetic heating plates, a vortex mixer, and a microcentrifuge for solution preparation and sample handling

The lab also houses a Tuttnauer 2840EL autoclave for sterilizing laboratory equipment and solutions.


Proper training is required before using this high-pressure device. Training is provided by RNDr. František Šťáhlavský, Ph.D. (stahlf@natur.cuni.cz)

Access to the lab:
New users always need to contact Pavel Munclinger and register with her before working in the laboratory. Please, make sure that everyone from your group (students, postdocs, and staff members) who works in the laboratory is registered. The lab has chip access. You can have it installed on your ISIC / ITIC card if you fill in a request form for room 202 at Viničná 7 here: http://web.natur.cuni.cz/www/intranet/formulare/form_cip.php.
You need to generate it in PDF format, print it, and get it signed by Pavel Munclinger and your department head. Once your document has all signatures, deliver it to CIT (Information Technology Division). You can use the faculty post to send it there.